Generally, we found a slight but not statistically significant increase on the mean values of genetic diversity parameters comparing the managed stand (HE = 0.410 A = 2.67) to the unmanaged stand (HE = 0.437 A = 2.33). Besides, we collected leaf material of 275 adult and regenerating individuals in the managed (213) and unmanaged stands (62), 60 months after the management. Subsequently, we used six SSR primers to quantify the genetic variability and spatial genetic structure of a stand under forest management and a natural stand.
In general, these primers revealed as an efficient tool for studies about genetic conservation and forest breeding E. The value of polymorphic information content (PIC) ranged from 0.438 to 0.943 and the observed (HO) and expected (HE) heterozygosity values ranged from 0.647 to 1.00 and 0.308 to 1.00 respectively. We identified 16 primers where 9 were polymorphic and satisfactorily amplified, producing 38 alleles with a mean of 4.22 alleles per primer. Afterwards, leaf material was collected from 42 individuals distributed in two forest fragments located in the southern region of the state of Minas Gerais. Firstly, for the development of the primers, we created an enriched microsatellite library for E erythropappus. One of the main approaches used in this study is the detection of possible genetic discontinuity between managed and unmanaged stands. erythropappus (b) analyze the spatial genetic structure and the kinship structure of regenerating and adult individuals (c) describe the levels of genetic diversity within and among managed and unmanaged stands. Specifically, we aim to: (a) develop and characterize nine microsatellite primers for E. The main objective of the present study is to analyze the effect of forest management under a silvicultural system of seed-trees on the genetic diversity and spatial genetic structure of Eremanthus erythropappus in Itamonte, Minas Gerais state. The detection of any genetic discontinuity is essential for the improvement of current management systems applied to tree species and for the definition of the cutting cycle. Understanding the genetic diversity and spatial genetic structure of tree species, as well as knowing about ecological and environmental factors related to their development are essential steps for applying appropriate management strategies for the genetic conservation. With the assumption that these three "subpopulations" were genetically identical before logging, this may indicate that a single logging event under the Malayan Uniform System (MUS) of harvesting practice did not cause genetic erosion in P. speciosa from the two regenerated stands were not significantly different from the control site. The t-tests showed that the overall genetic diversity measures of P.
RAPD analyses using seven 10-mer arbitrary primers yielded a total of 51 consistent loci. speciosa trees from three adjacent forest management units in Pasoh Forest Reserve, i.e., the Unlogged Stand (control site), Regenerated Stand 1 (logged in 1951) and Regenerated Stand 2 (logged in 1955) were analysed. Random amplified polymorphic DNA (RAPD) markers were used to estimate the genetic diversity parameters, namely, Shannon's diversity index, mean number of alleles per locus, effective number of alleles per locus, Nei's gene diversity and percentage of polymorphic loci. This study examined the effects of a single logging event (taken place more than 40 years ago) on the genetic diversity of Parkia speciosa in a lowland dipterocarp forest.
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